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Effects of dietary mannanase on growth, metabolism and non‐specific immunity of T ilapia ( O reochromis niloticus )
Author(s) -
Chen Wenyan,
Lin Shimei,
Li Fajian,
Mao Shuhong
Publication year - 2016
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12733
Subject(s) - biology , feed conversion ratio , oreochromis , cholesterol , transaminase , very low density lipoprotein , alanine transaminase , protein efficiency ratio , aspartate transaminase , amylase , lysozyme , tilapia , digestive enzyme , zoology , medicine , endocrinology , lipoprotein , biochemistry , enzyme , alkaline phosphatase , body weight , fish <actinopterygii> , fishery
An experiment was designed to assess the effects of a commercial β‐mannanase on performance and immunity of tilapia fed plant‐based diets. A basal diet was supplemented with 0.0 (control), 0.5 and 1.0 g β‐mannase kg −1 to formulate three experimental diets. Each treatment contained 4 tanks with 30 fish per tank. Trial lasted 8 weeks. Our results demonstrated that β‐mannanase addition (0.5 and 1.0 g kg −1 ) improved significantly ( P < 0.05) the final weight, specific growth rate ( SGR ), protein efficiency ratio ( PER ) and feed conversion ratio ( FCR ) compared with the control (0.0 g kg −1 ). There were no significant differences in feed intake ( FI ) and survival rate ( SR ) among the 3 dietary treatments ( P > 0.05). β‐mannanase supplementation also led to an increase ( P < 0.05) in amylase, trypsin and Na + K + ‐ ATP ase activities in intestine, and an decrease ( P < 0.05) in aspartate transaminase ( AST ) and alanine transaminase ( ALT ) activities in serum compared with the control. However, dietary enzyme supplementation had no significant effect on the serum triacylglycerol ( TG ), cholesterol ( CHO ), high density lipoprotein cholesterol ( HDL ‐C), low density lipoprotein cholesterol ( LDL ‐C) and very low density lipoprotein cholesterol ( VLDL ‐C) ( P > 0.05). Moreover, the dietary β‐mannanase supplementation groups exhibited an increase in the total leukocyte counts ( WBC ), differential leukocyte counts, respiratory burst activity, lysozyme activity and superoxide dismutase ( SOD ) activity compared to the controls ( P < 0.05). In conclusion, β‐mannanase addition to tilapia diets improved feed utilization and non‐specific immunity resulting in improvements in growth performance.