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Dietary probiotic supplementation in juvenile rainbow trout ( Oncorhynchus mykiss ) reared under cage culture production: effects on growth, fish welfare, flesh quality and intestinal microbiota
Author(s) -
Ozório Rodrigo O A,
KopeckaPilarczyk Justyna,
Peixoto Maria J,
Lochmann Rebecca,
Santos Ricardo J,
Santos Gonçalo,
Weber Barbara,
Calheiros Joaquim,
FerrazArruda Lia,
VazPires Paulo,
Gonçalves José F M
Publication year - 2016
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12724
Subject(s) - biology , probiotic , rainbow trout , food science , glutathione peroxidase , zoology , prebiotic , trout , lysozyme , antioxidant , fishery , bacteria , biochemistry , catalase , fish <actinopterygii> , genetics
This study was conducted to evaluate the effects of dietary probiotic supplementation on growth performance, fish welfare, sensory evaluation, freshness during ice storage and changes in intestinal microbiota of rainbow trout ( Oncorhynchus mykiss ). The 9‐week feeding trial was conducted in cages under commercial conditions during the winter season, when water temperature decreased from 14°C to 7°C. Fish were fed either a diet without probiotic supplementation (control) or with 0.2% (2 × 10 9 CFU kg −1 diet) of multi‐strain probiotic bacteria ( Bacillus sp., Pediococcus sp., Enterococcus sp., Lactobacillus sp.). Fish weight was recorded at 0, 2, 4, 6 and 9 weeks. Growth performance was not affected by dietary probiotic supplementation. Alternative complement activity ( ACH 50) was significantly higher in fish fed the diet with probiotic (58.4 ± 3.7 units mL −1 ) than in the control group (46.9 ± 3.1 units mL −1 ) ( P = 0.03). Several biochemical markers were altered by the dietary treatment. 7‐ethoxyresorufin‐O‐deethylase ( EROD ) ( P < 0.01), glutathione S‐transferase ( GST ) ( P < 0.001), glutathione peroxidase ( GP x) ( P < 0.05) increased and lipid peroxidation ( LP ) ( P < 0.01) decreased with dietary probiotic supplementation. Flesh quality and shelf‐life of fish stored on ice was not affected by dietary probiotic supplementation. Probiotic supplementation had a limited effect on the intestinal microbiota. Signal strength of the observed operational taxonomic units ( OTU s) differed between the control and treatment samples and could indicate variations in the abundance of certain OTU s. Microbiota adhering to the intestinal mucus varied between sampling points, indicating that changes were associated with water temperature.