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Comparative effect of Freund's adjuvant and Aloe vera L. gel on the expression of TNF‐α and IL‐1β in vaccinated Cyprinus carpio L. with Aeromonas hydrophila C. bacterin
Author(s) -
Abdy Esmail,
Alishahi Mojtaba,
Tabandeh Mohammad Reza,
Ghorbanpoor Masoud,
Jafari Hadis
Publication year - 2016
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12702
Subject(s) - aloe vera , aeromonas hydrophila , cyprinus , carp , biology , adjuvant , common carp , aeromonas , immune system , microbiology and biotechnology , immunology , fish <actinopterygii> , fishery , bacteria , genetics
The comparative effects of Freund's and Aloe vera gel as adjuvants on the expression of IL ‐1β and TNF ‐α genes were studied in vaccinated common carp ( Cyprinus carpio ) with Aeromonas hydrophila bacterin. Fishes were intraperitoneally immunized with A. hydrophila bacterin in combination with Aloe vera gel or Freund's and also without any adjuvant. At day 28 after immunization, all groups were challenged by lethal dose of A. hydrophila (10 7 cells/fish). Changes in the expression of IL ‐1β and TNF ‐α genes were evaluated in anterior kidney before challenge and 12, 24, 72 and 7 days postchallenge using quantitative real‐time PCR . Higher expression levels of both genes were observed in all vaccinated groups compared with non‐immunized group. Fishes which received Aloe vera gel showed higher expression of IL ‐1β and TNF ‐α in relation to animals which vaccinated with or without Freund's adjuvant. We concluded that Aloe vera gel in compared with Freund's adjuvant had a more stimulatory effect on the expression of immune‐related genes in vaccinated common carp and it can use as a novel adjuvant in aquaculture.