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Molecular cloning, tissue distribution and nutritional regulation of a fatty acyl elovl5‐like elongase in large yellow croaker, Larimichthys crocea
Author(s) -
Zuo Rantao,
Mai Kangsen,
Xu Wei,
Dong Xiaojing,
Ai Qinghui
Publication year - 2016
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12686
Subject(s) - biology , complementary dna , untranslated region , open reading frame , cloning (programming) , polyunsaturated fatty acid , molecular cloning , rapid amplification of cdna ends , biochemistry , messenger rna , fatty acid , microbiology and biotechnology , reverse transcriptase , gene , peptide sequence , polymerase chain reaction , computer science , programming language
In this study, the full‐length cDNA of fatty acyl elovl5‐like elongase was first cloned from large yellow croaker, Larimichthys crocea . The cDNA of elovl5‐like was 1551 bp, including a 5′‐terminal untranslated region ( UTR ) of 120 bp, a 3′‐terminal UTR of 546 bp and an open reading frame ( ORF ) of 885 bp encoding a polypeptide of 294 amino acid residues. Sequence comparison showed that the predicted protein revealed a high percentage identity (>80%) with Elovl5 from other marine fish species. Tissue distribution analysis revealed that the elovl5‐like was expressed highly in liver, brain and gill, and at much lower levels in stomach, intestine, heart and spleen. Quantity polymerase chain reaction showed that hepatic elovl5‐like transcription decreased significantly with the increase in dietary n‐3 LC ‐ PUFA ( P < 0.05). The mRNA levels of elovl5‐like in the liver of fish fed diets with 0.15%, 0.60% and 0.98% n‐3 LC ‐ PUFA were up‐regulated by 1.77‐fold, 1.41‐fold and 1.41‐fold than that with 2.25% n‐3 LC ‐ PUFA respectively. No significant differences were observed in the hepatic mRNA levels of elovl5‐like in response to the increased ratio of dietary DHA / EPA ( P > 0.05). These results demonstrate for the first time the presence and nutritional modulation of elovl5‐like cDNA in large yellow croaker. This could contribute to better understanding the process of n‐3 LC ‐ PUFA biosynthesis in this fish species.