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Protective efficacy of active compounds from P hyllanthus amarus against white spot syndrome virus in freshwater crab ( P aratelphusa hydrodomous )
Author(s) -
Sundaram Dinesh,
Kesavan Karthikeyan,
Kumaravel Hemalatha,
Mohammed Roohi Fatima,
Tohru Mekata,
Toshiaki Itami,
Raja Sudhakaran
Publication year - 2016
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12660
Subject(s) - white spot syndrome , biology , shrimp , bioassay , petroleum ether , traditional medicine , virus , virology , veterinary medicine , fishery , chromatography , ecology , medicine , chemistry , extraction (chemistry)
Repeatedly the shrimp culturing industry faces serious economic loss due to white spot disease caused by white spot syndrome virus ( WSSV ) in penaeid shrimp. The present study was carried out to determine the antiviral activity of traditional I ndian medicinal herb P hyllanthus amarus against WSSV in freshwater crab, P aratelphusa hydrodomous ( H erbst). For experimental challenge, crabs were injected with three different solvent extracts of P . amarus (aqueous, acetone and petroleum ether) along with WSSV . The efficacy of the extracts was confirmed by bioassay, histopathology and reverse transcriptase – polymerase chain reaction ( RT ‐ PCR ). The overall results of the present study confirmed that acetone and petroleum ether extracts of P . amarus have significant antiviral effect against WSSV .