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Insights on the association between somatic aneuploidy and ostreid herpesvirus 1 detection in the oysters Crassostrea gigas , C. angulata and their F1 hybrids
Author(s) -
Batista Frederico M,
LópezSanmartín Monserrat,
Boudry Pierre,
Navas José I,
Ruano Francisco,
Renault Tristan,
Fonseca Vera G,
Leitão Alexandra
Publication year - 2016
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12613
Subject(s) - biology , crassostrea , pacific oyster , oyster , aneuploidy , somatic cell , ostreidae , genetics , virology , microbiology and biotechnology , gene , aquatic animal , shellfish , ecology , fishery , fish <actinopterygii> , chromosome
Cytogenetic abnormalities associated with viral infections, including from viruses of the Herpesvirales order, have been reported in vertebrate species. Ostreid herpesvirus 1 (Os HV ‐1) has been detected worldwide during mortality outbreaks of the Pacific oyster Crassostrea gigas . On the other hand, a high proportion of aneuploid cells in somatic tissues have been observed in C. gigas . In this study, we analysed the putative association between aneuploidy levels and the detection of Os HV ‐1 in gills of C. gigas , the Portuguese oyster C. angulata and their F1 hybrids cultured in Ria Formosa (Portugal). Os HV ‐1 was detected by PCR in 5.4% of the total of oysters analysed ( n = 111) namely in 11.1%, 8.0% and 1.7% of C. gigas , C. angulata and F1 hybrid respectively. Sequencing analysis of a viral fragment amplified with the C2/C6 primer pair revealed a high similarity with the Os HV ‐1 reference type. Moreover, in situ hybridization confirmed the presence of Os HV ‐1 in gill tissue. Oysters where Os HV ‐1 was detected had a significantly higher mean percentage of aneuploid cells (25%) than the ones where the virus was not detected (18%). However, the overall low percentage of positive samples contrasted with the high mean percentage of aneuploidy observed, with 50% of the oysters analysed showing a percentage of aneuploid cells between 20% and 30%. We hypothesize that somatic aneuploidy may adversely affect oysters making them more prone to Os HV ‐1 infection, but the virus is unlikely to be the cause of somatic aneuploidy.