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Oral administration of Escherichia coli lipopolysaccharide enhances the immune system of striped catfish, Pangasianodon hypophthalmus (Sauvage)
Author(s) -
Bich Hang Bui Thi,
Nguyen Phuong Thanh,
Kestemont Patrick
Publication year - 2016
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12589
Subject(s) - catfish , biology , edwardsiella ictaluri , lysozyme , immunostimulant , lipopolysaccharide , microbiology and biotechnology , immune system , respiratory burst , escherichia coli , edwardsiella tarda , antibody , immunity , zoology , fish mortality , veterinary medicine , fish <actinopterygii> , immunology , fishery , biochemistry , gene , medicine , ictalurus
Abstract Lipopolysaccharide ( LPS ), a component of Gram negative bacteria, was reported as important immunostimulant for fish. In this study, striped catfish were fed diets containing different Escherichia coli LPS concentrations (0%, 0.01% and 0.05%) for 2 weeks and then fed control feed (0% LPS ) for 4 weeks. Plasma cortisol and glucose were rather low and did not differ significantly among treatments ( P > 0.05). The respiratory burst activity, lysozyme, complement, total of antibody as well as mortality in fish challenged with Edwardsiella ictaluri were recorded every 2 weeks (W2, W4 and W6). The lysozyme activity significantly increased in fish treated with LPS ( P < 0.05) in W2, W4 and W6. The highest values of respiratory burst activity were observed at week 4 in fish fed 0.01% LPS . There were significant differences in total of antibody between fish fed LPS (0.01%) and control in W2, W4. The challenge test with Edwardsiella ictaluri showed that fish fed 0.01% LPS had lower cumulative mortality (40%, 33% and 42%) compared with the fish fed 0.05% LPS (50%, 40% and 47%) and control fish (40%, 57% and 53%) in the three difference sampling times respectively. These results suggest that feed supplemented with 0.01% LPS could enhance immunity of striped catfish after 2 weeks of oral administration and fish could be protected against bacterial infection during the following 4 weeks.