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Immunostimulatory effects of N ‐Oxide–Quaternary alkaloid fraction of a marine C hlorophycean macroalga in the striped murrel, C hanna striata ( B loch)
Author(s) -
Balasubramanian Ramalakshmi,
Michael R Dinakaran
Publication year - 2016
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12518
Subject(s) - aeromonas hydrophila , biology , alkaloid , immune system , peroxidase , virulence , lysozyme , microbiology and biotechnology , veterinary medicine , enzyme , immunology , biochemistry , botany , fishery , gene , medicine , fish <actinopterygii>
N ‐Oxide–Quaternary Alkaloid Fraction ( NOQAF ) of a C hlorophycean macroalga, C aulerpa scalpelliformis (R.Br.) C. Ag f. denticulata (Deaisne) Weber van Bosse (Patent filed) was intraperitoneally administered at different doses (0, 2, 20 or 200 mg kg −1 body weight) in different groups of C hanna striata to assess its effect on the non‐specific immune responses and disease resistance against live virulent A eromonas hydrophila . A reference positive control [ M acroGard ( B iotec P harmacon ASA , N orway) 20 mg kg −1 body weight] was simultaneously maintained to compare its efficacy. The fraction ( NOQAF ) has been found to enhance the serum lysozyme, peroxidase, antiprotease and alternate complement activities and the reactive oxygen and nitrogen species production and peroxidase activity by the peripheral blood leucocytes than the control group during the study period. On experimental challenge with live virulent A . hydrophila, maximum RPS values of 88.8 and 87.4 were noted in the groups treated with single dose of 20 mg kg −1 and double dose of 2 mg kg −1 of NOQAF respectively. In conclusion, the intraperitoneal administration of the N ‐Oxide–Quaternary Alkaloid Fraction ( NOQAF ) of C . scalpelliformis has been found to significantly enhance the non‐specific immune responses and disease resistance against A . hydrophila in C hanna striata .