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Protein‐sparing effects and LPL gene expressions of dietary lipids in the juvenile soft‐shelled turtle, Pelodiscussinensis
Author(s) -
Tang Jing,
Wang AnLi,
Miao YuTao,
Zhou YuanHang,
Luo MinQi,
Xiao PeiZhen
Publication year - 2016
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12517
Subject(s) - biology , fish oil , lipoprotein lipase , fish meal , lipase , hepatic lipase , corn oil , juvenile , soybean oil , food science , zoology , medicine , endocrinology , biochemistry , enzyme , fish <actinopterygii> , fishery , ecology
The aim of this study was to evaluate the effects of dietary lipids on protein‐sparing and lipoprotein lipase ( LPL ) m RNA expression in culture using 360 juvenile soft‐shelled turtles ( P elodiscussinensis ) (initial weight 4.26 ± 0.14 g). The turtles were allotted to six diets with three duplicates for 60 days. A control diet with 46% protein and 55% fishmeal ( CD ) and five isonitrogenous diets with 41.3% protein and 45% fishmeal (F, S, L1, L2 and L3) were used, containing the following three lipid types: fish oil, soybean oil and mixed oils (soybean oil: fish oil = 1:1). The results showed that the survival rate was not affected by dietary lipids ( P > 0.05). The highest weight gain and lowest feed coefficient ratio were seen in the L3 diets ( P < 0.05). Turtles fed with L2 and L3 diets had lower superoxide dismutase activities, higher alanine aminotransferase activities and higher cholesterol concentrations than those exposed to other diets ( P < 0.05). Hepatic LPL activity and LPL m RNA expression were higher in the L3 diets than in the other diets ( P < 0.05). Overall, there were obvious protein‐sparing effects of dietary lipids and LPL m RNA expression was stimulated by high dietary lipids in soft‐shelled turtles in this study.