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Biomass production, proximate composition and fatty acid profile of the local marine thraustochytrid isolate, S chizochytrium sp. LEY 7 using low‐cost substrates at optimum culture conditions
Author(s) -
LudevesePascual Gladys,
Dela Peña Milagros,
Tornalejo Jilla
Publication year - 2016
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12494
Subject(s) - biology , food science , biomass (ecology) , polyunsaturated fatty acid , yeast extract , fatty acid , eicosapentaenoic acid , palmitic acid , fermentation , botany , biochemistry , ecology
This study was conducted to investigate low‐cost substrate alternative and the optimum culture conditions in mass producing the local marine thraustochytrid, S chizochytrium sp. LEY 7 isolated from the mangrove leaves of B aybay, S outhern L eyte P hilippines. Results showed that S chizochytrium sp. LEY 7 is able to utilize commercial grade glucose and yeast extract from NaCl ‐treated baker's yeast as source of carbon and micronutrients respectively. Cost of mass producing the thraustochytrid isolate using the alternative production substrates was substantially reduced. Incubation temperature and salinity levels were the two growth factors significantly affecting the biomass production of the isolate. The short duration of lag phase shown by the isolate suggests a growth advantage in that cells are readily able to adapt to their new environment. Total lipids averaged to 19.4%. Principal fatty acids were palmitic acid (C16:0) with 33.52% and docosahexanoic acid ( DHA , C22:6n‐3) with 39.92% proportion to total fatty acid. Eicosapentaenoic (C20:5n‐3, 1.01%) and arachidonic (C20:4n‐3, 0.90%) are present but in relatively lower amount. Our findings showed the potential of low‐cost substrate in mass producing the local thraustochytrid isolate, S chizochytrium sp. LEY 7 as lipid and polyunsaturated fatty acid source in aquaculture. Biomass production was enhanced by optimizing the culture conditions.