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Effects of dietary levan on growth performance, nonspecific immunity, pathogen resistance and body composition of orange‐spotted grouper ( E pinephelus coioides H.)
Author(s) -
Huang MeiYing,
Chang ChinI,
Chang ChihChien,
Tseng LiangWei,
Pan ChorngLiang
Publication year - 2015
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12430
Subject(s) - grouper , biology , epinephelus , lysozyme , orange (colour) , zoology , food science , microbiology and biotechnology , veterinary medicine , fishery , biochemistry , fish <actinopterygii> , medicine
This study investigated the effects of levan produced by B acillus licheniformis FRI MY ‐55 on growth performance, intestinal count of viable bacteria, immune status, pathogen resistance and body composition of orange‐spotted grouper ( E pinephelus coioides ). Orange‐spotted grouper were fed diets supplemented with levan at concentrations of 0 (control), 5.0, 10.0, 25.0 and 50.0 g kg −1 for 12 weeks. The final weight and per cent weight gain were significantly higher in the 25.0 g kg −1 levan‐supplemented group than in the control group ( p < 0.05). All levan‐supplemented diets significantly decreased the count of total viable aerobic bacteria and Vibrio spp. in the intestines of groupers ( p < 0.05). Serum total protein levels, globulin levels, lysozyme activity and survival rate of orange‐spotted grouper after challenge with V. harveyi were significantly higher in the 25.0 g kg −1 levan‐supplemented group than in the control group ( p < 0.05). This study also found that the 10.0, 25.0 and 50.0 g kg −1 levan‐supplemented diets significantly increased the crude protein level in the body composition of orange‐spotted grouper. Overall, the results of this study indicate that dietary levan (25.0 g kg −1 ) could be an effective method for enhancing the growth performance and disease resistance in orange‐spotted grouper.