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Improvement in wild endangered P ersian sturgeon, A cipenser persicus ( B orodin, 1897) semen cryopreservation by 2‐hydroxypropyl‐beta‐cyclodextrin (Hβ CD )
Author(s) -
Rahimi Ruhollah,
Farahmand Hamid,
Mirvaghefi Alireza,
Rafiee Gholamreza,
Abed Elmdoust Amirreza
Publication year - 2015
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12403
Subject(s) - acipenser , semen , cryopreservation , biology , cryoprotectant , motility , extender , semen quality , andrology , zoology , sperm motility , liquid nitrogen , semen cryopreservation , sturgeon , anatomy , fishery , chemistry , fish <actinopterygii> , medicine , embryo , genetics , organic chemistry , polyurethane
In this study, cryopreservation feasibility of P ersian sturgeon ( A cipenser persicus ) and the effect of different doses of 2‐hydroxypropyl‐beta‐cyclodextrin on thawed spermatozoa quality (motility duration and motility percentage) were investigated. For freezing, semen of seven male individuals was pooled in equal volumes and diluted with 4°C [Tris‐HCl (100 mM), pH = 8, DMSO 10%] extenders containing 0, 5, 10, 15 mM of Hβ CD in a ratio of 1:1(semen/extenders). Then semen was filled into 0.5‐mL straws, and was frozen with vapour of liquid nitrogen at 4‐cm above surface of liquid nitrogen. After 3 min, straws were plunged in to liquid nitrogen. Thawing was performed at 40°C water baths for 15 s. Motility duration of the 10 mM Hβ CD treated spermatozoa at days 14 (228.98 ± 16.39) and 56 (199.66 ±21.78) were longer than other treatments. In day 56, the motility percentage in treatment with 10 mM was significantly higher (16.14 ± 2.54) ( P < 0.05) compared with 5 mM treatment (8.75 ± 2.47) ( P < 0.05). Therefore, it is recommended that 10 mM of Hβ CD can be used as an additive cryoprotectant for increasing cryopreserved spermatozoa quality in this species.