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A set of 13 multiplex PCR s of specific microsatellite markers as a tool for QTL detection in gilthead seabream ( S parus aurata L.)
Author(s) -
NegrínBáez Davinia,
Navarro Ana,
LeeMontero Ivonne,
Afonso Juan M,
Sánchez Juan J,
Elalfy Islam S,
Manchado Manuel,
Sánchez José A,
GarcíaCeldrán Marta,
Zamorano María J
Publication year - 2015
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12378
Subject(s) - biology , microsatellite , multiplex , genotyping , quantitative trait locus , genetics , multiplex polymerase chain reaction , allele , marker assisted selection , loss of heterozygosity , locus (genetics) , genetic marker , gene , genotype , polymerase chain reaction
The growth and consolidation of the gilthead seabream ( S parus aurata ) industry require improvement based on permanent and cumulative aspects, such as those derived from genetic breeding programmes. Marker Assisted Selection ( MAS ) by Quantitative Trait Loci ( QTL ) can be usefully implemented with the appropriate tools. In this study, 138 microsatellite markers from the genetic map of gilthead seabream were redesigned to be amplified under the same PCR conditions. A final set of 13 multiplex PCR s (named ReM sa ) with 106 of these markers was developed to cover 100% of the linkage groups. These effective multiplex PCR s enable to optimize QTL searching with a critical reduction in costs and errors. Results showed that the mean value of the number of alleles for 106 markers was 6.9. The mean observed heterozygosity ranged from 0.53 to 0.86, and 74.5% of markers were highly informative according to their polymorphic information content. The correct inheritance and segregation of alleles of each locus was confirmed after genotyping 62 individuals of a full‐sib family by these multiplex PCR s. In addition, genetic features of the 20 microsatellite markers that worked correctly but were not included in any multiplex PCR are also reported to provide geneticists with the possibility of including them in more comprehensive screening studies.