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Using B acillus subtilis E20‐fermented soybean meal as replacement for fish meal in the diet of orange‐spotted grouper ( E pinephelus coioides , H amilton)
Author(s) -
Shiu YaLi,
Hsieh ShuLing,
Guei WangChen,
Tsai YingTsai,
Chiu ChiuHsia,
Liu ChunHung
Publication year - 2015
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12294
Subject(s) - grouper , biology , fish meal , digestive enzyme , soybean meal , feed conversion ratio , zoology , meal , epinephelus , food science , amylase , fishery , biochemistry , enzyme , ecology , endocrinology , fish <actinopterygii> , body weight , raw material
Summary The potential of B acillus subtilis E20‐fermented soybean meal ( FSBM ) as a partial alternative component of fish meal ( FM ) in fed diets of orange‐spotted grouper ( E pinephelus coioides) was evaluated in this study. An FM ‐based diet and seven diets containing 10%, 20% and 30% and 10%, 20%, 30% and 40% of FM replaced by soybean meal ( SBM ) and FSBM , respectively, were fed to grouper for 84 days to evaluate possible substitution levels of FM by tracking growth performance, digestive enzyme activity and morphological changes in the liver and distal intestine. No significant differences in survival and muscle composition of grouper were found between controls and treatments. Growth performance and feed efficiency of fish fed diets with FM replaced by FSBM up to 30% were not significantly different from controls, whereas significantly decreased growth performance and feed efficiency occurred with diets containing >20% of SBM . Based on the feed efficiency, the maximum substituted levels of FM by SBM and FSBM in grouper diets were 18.36% and 29.32%, respectively, based on broken‐line analyses. Histopathological changes in the liver and distal intestine, and significantly lower activity levels of digestive enzymes, including pepsin in the stomach and trypsin, chymotrypsin, amylase and lipase in the distal intestine, were found in fish fed a diet containing 30% of FM replaced by SBM . However, these parameters were improved by the substitution of FSBM . It is therefore believed that FSBM has great potential to be used as a protein source in grouper diets in partial replacement of FM .