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In vitro and in vivo antifungal activity of S atureja cuneifolia ten essential oil on S aprolegnia parasitica strains isolated from rainbow trout ( O ncorhynchus mykiss , Walbaum) eggs
Author(s) -
Metın Secil,
Dıler Oznur,
Dıdınen Behire Isıl,
Terzıoglu Sedef,
Gormez Oznur
Publication year - 2015
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12293
Subject(s) - biology , hatching , rainbow trout , incubation , incubation period , micafungin , satureja , essential oil , saprolegnia , zoology , microbiology and biotechnology , botany , antifungal , amphotericin b , biochemistry , fishery , fish <actinopterygii>
In the present study, the chemical composition and the antifungal properties against S aprolegnia parasitica ( in vitro and in vivo ) of the essential oils of thyme ( S atureja cuneifolia ) from M editerranean region of T urkey were evaluated for the first time. The composition of oils was analysed using gas chromatography/mass spectrometry ( GC / MS ). The major constituents of oil of S . cuneifolia were cavracrol (46,84%) and cymene (16.90%). Antifungal effects of S . cuneifolia essential oil against S . parasitica strains (A1 and E1) were detected by disc diffusion and tube dilution assays. The antifungal effect of S . cuneifolia was determined to be stronger against S . parasitica E1 isolate ( MIC 50 μL mL −1 , MLC 250 μL mL −1 ) compared with S . parasitica A1 isolate ( MIC 50 μL mL −1 , MLC 500 μL mL −1 ). Following in vitro assays, effective doses of S . cuneifolia for disease control in rainbow trout eggs experimentally infected with S . parasitica were investigated. For this aim, infected eggs were treated with the essential oil (0, 5, 10, 20 and 50 ppm) during incubation period (21 days) after fertilization. Formalin (5 mL L −1 ) was used as positive control. Hatching rate of eggs at the end of incubation period were calculated. The highest hatching rates were recorded in S . parasitica E1 strain at 5 and 10 ppm concentrations of S . cuneifolia and in S . parasitica A1 strain at 10 and 20 ppm ( P < 0.05).