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Bacterial community of pond's water, sediment and in the guts of tilapia ( O reochromis niloticus ) juveniles characterized by fluorescent in situ hybridization technique
Author(s) -
Del'Duca Alessandro,
Cesar Dionéia Evangelista,
Abreu Paulo César
Publication year - 2015
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12218
Subject(s) - biology , oreochromis , tilapia , lactobacillus , pseudomonas fluorescens , aquaculture , microbiology and biotechnology , probiotic , oreochromis mossambicus , bacteria , veterinary medicine , fishery , fish <actinopterygii> , medicine , genetics
Information about bacterial community structure and functioning in fish farming ponds remains scarce, mainly due to methodological difficulties in counting and identifying uncultured bacteria. The main objective of this study was to evaluate the degree of similarity between the bacterial community of the digestive tract of tilapia ( O reochromis niloticus ) juveniles and that of the test pond's water and sediment, using the Fluorescent In Situ Hybridization ( FISH ) technique. Samples of water, sediment and gut content of 30 tilapia juveniles from a single nursery ground were collected in January 2010. Potentially probiotic and pathogenic bacteria of the species Bacillus , L actobacillus brevis , L actobacillus collinoides , Lactobacillus coryniformis , L actobacillus farciminis , Vibrio and P seudomonas fluorescens were found in different samples using specific fluorescent probes. The similarity between bacterial community environments and gastrointestinal tracts was determined using the Morisita‐Horn index. The fish guts presented higher abundances of P seudomonas fluorescens , Bacillus , L actobacillus brevis and L actobacillus collinoides . The bacterial community composition of tilapia gastrointestinal tract was more similar to the water than the sediment of the pond. The results of this study showed that the FISH technique can be easily used for monitoring of probiotics and pathogen detection in aquaculture systems.

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