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Biomarkers of oxidative stress and antioxidant defences as indicators of different disinfectants exposure in the heart of rainbow trout ( Oncorhynchus Mykiss Walbaum)
Author(s) -
Tkachenko Halyna,
Kurhaluk Natalia,
Grudniewska Joanna
Publication year - 2015
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12214
Subject(s) - tbars , oxidative stress , antioxidant , superoxide dismutase , glutathione peroxidase , glutathione reductase , catalase , chlorine dioxide , rainbow trout , glutathione , thiobarbituric acid , chloramine , food science , chemistry , biochemistry , biology , microbiology and biotechnology , lipid peroxidation , enzyme , fishery , chlorine , fish <actinopterygii> , inorganic chemistry , organic chemistry
The aim of this study was to evaluate the impacts of different disinfectants' treatment using in aquaculture on the oxidative stress biomarkers such as thiobarbituric acid reactive substrates ( TBARS ) and carbonyl derivatives of protein oxidative modification, as well as antioxidant defences [superoxide dismutase ( SOD ), catalase ( CAT ), glutathione reductase ( GR ) and glutathione peroxidase ( GPx ) activity] and total antioxidant capacity ( TAC ) in the heart tissue of rainbow trout ( Oncorhynchus mykiss ). In the disinfectants exposure, fish were treated to chloramine‐ T (final concentration 10 g m −3 ), chloride dioxide (5 g m −3 ), formalin (200 mL m −3 ) and CIP disinfectant based on peracetic acid and hydrogen peroxide (16 mL m −3 ) for 20 min and repeated three times every 3 days. Both chlorine dioxide and formalin treatment was indicated by a significant increase in the level of heart TBARS levels and carbonyl derivatives content and decreased SOD activity. Tissue oxidative stress biomarkers were unchanged upon chloramine‐ T or CIP disinfectant exposure. Increased oxidative stress could modify antioxidant defences, principally causing increased CAT activity in the heart tissue of formalin‐ or ClO 2 − ‐exposed fish. The correlation between oxidative stress biomarkers and GPx activity indicates that enzymes related to glutathione metabolism were responsible to formalin or ClO 2 − ‐induced oxidative stress. Hence, TBARS , carbonyl derivatives and antioxidant defences could be used as biomarkers in evaluating the toxicity of formalin and chlorine dioxide using as disinfectants to trout.