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Physiological responses and HSP 70 m RNA expression of GIFT strain of N ile tilapia ( O reochromis niloticus ) under cold stress
Author(s) -
Shi GuiCheng,
Dong XiaoHui,
Chen Gang,
Tan BeiPing,
Yang QiHui,
Chi ShuYan,
Liu HongYu
Publication year - 2015
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12212
Subject(s) - nile tilapia , oreochromis , biology , hsp70 , tilapia , catalase , lactate dehydrogenase , superoxide dismutase , antioxidant , triglyceride , cholesterol , endocrinology , medicine , enzyme , biochemistry , heat shock protein , fishery , fish <actinopterygii> , gene
Abstract An investigation on the physiological responses and HSP 70 expression under cold stress was conducted on Genetically Improved Farmed Tilapia ( GIFT ) strain of N ile tilapia ( O reochromis niloticus ). Tilapia were acclimated at 28°C as control and then the water temperature was reduced from 28°C to 15°C at a rate of 1°C h −1 , and serum biochemical indices, antioxidant enzymes and expression of HSP 70 m RNA were analysed on days 0, 0.5, 1, 2, 3 and 5 after exposure to 15°C. The concentration of serum K , Na , Cl and Ca concentration showed instability during cold stress. Glucose rapidly increased on day 0 followed by a declining trend. Triglyceride, cholesterol, low density lipoprotein‐cholesterol signifi‐cantly decreased and high density lipoprotein‐cholesterol showed instability during cold stress. Aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase activities were prominently elevated under cold stress. Superoxide dismutase and catalase activities showed a remarkable rise on day 0.5 under cold stress, and gradually decreased thereafter. HSP 70 m RNA level significantly increased both in liver and muscle under cold stress, especially on days 0.5 and 1. These results suggest that cold influences several physiological responses of tilapia, and the cold resistance and cold tolerance of tilapia will benefit from the enhancement of antioxidant enzymes activities and upregulated HSP 70 m RNA expression.