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Innate immune response and gene expression of Scylla paramamosain under Vibrio parahaemolyticus infection
Author(s) -
Zhang Zhao,
Li Shengkang,
Xie Chuping,
Zhou Lizhen,
Li Chuanbiao,
Liu Wenhua,
Wen Xiaobo
Publication year - 2015
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12194
Subject(s) - scylla paramamosain , vibrio parahaemolyticus , biology , microbiology and biotechnology , superoxide dismutase , innate immune system , hepatopancreas , catalase , alkaline phosphatase , immune system , hemolymph , gene , bacteria , enzyme , ecology , biochemistry , immunology , genetics
An epidemic of ‘milky disease’ in the mud crab ( Scylla paramamosain ) generally breaks out in the fall when the crab is near maturity, resulting in large economic losses in crab farming. Vibrio parahaemolyticus has been proven to be one of the major pathogens. In this study, the mud crabs were challenged with V. parahaemolyticus , and their innate immune responses were investigated in terms of total haemocyte counts ( THC s), haemocytic enzyme activities and gene expression levels during a 114‐h period. The THC s of the mud crabs decreased significantly after 42 h of exposure. The activities of the haemocytic enzymes, including acid phosphatase‐alkaline phosphatase, phenoloxidase, superoxide dismutase (SOD), peroxidase and nitric oxidase synthethase, were significantly enhanced during the challenge course. The gene expression levels also significantly increased for all tested genes (pro PO , Cu/Zn‐ SOD , Prx, LYS , CRU and ALF ) with the exception of CAT down‐regulated expression. The results may imply that the immune responses of the mud crab could be activated by the pathogens, and the data here will provide many clues for further systematic investigation of ‘milky disease’ caused by V. parahaemolyticus and the disease prevention in mud crab S. paramamosain .