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Pathogenicity of halophilic V ibrio harveyi in giant freshwater prawns ( M acrobrachium rosenbergii De Man)
Author(s) -
Siripornadulsil Wilailak,
Thongserm Mutjarin,
Siripornadulsil Surasak
Publication year - 2014
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12144
Subject(s) - biology , lysogenic cycle , penaeus monodon , vibrio harveyi , macrobrachium rosenbergii , microbiology and biotechnology , virulence , vibrio , halophile , hepatopancreas , lysogen , shrimp , bacteria , prawn , ecology , bacteriophage , escherichia coli , gene , genetics
In this study, the phenotypic and pathogenic properties in freshwater host were characterized in 14 strains of halophilic V ibrio harveyi isolated from infected marine black tiger shrimp, P enaeus monodon . The lysogenic phenotype was assayed via prophage excision and mitomycin C induction. The bacteria were grouped into two types, corresponding to lysogenic and non‐lysogenic strains. The pathogenicity was determined via direct injection of bacterial cultures into post‐larval juvenile giant freshwater prawns, M acrobrachium rosenbergii De Man. All of the infected prawns showed similar symptoms and inflamed hepatopancreas. The V . harveyi isolates derived from the first‐injected infected prawns were re‐isolated and re‐injected into healthy giant freshwater prawns, in which they retained similar infectivity. Both lysogenic and non‐lysogenic Vibrio spp. showed identical virulence associated with 100% mortality within one day post‐injection. TEM micrographs showed hepatopancreatic nuclear deformation and lipid breakdown caused by lysogenic γ‐hemolytic VL 19 and non‐lysogenic β‐hemolytic V33. However, the V33 strain was associated with severely disrupted mitochondria. None of the V . harveyi strains was able to produce a biofilm. Together, our findings indicate that the lysogenic and non‐lysogenic halophilic V . harveyi isolated from marine shrimps may use different virulence factors that are responsible for their pathogenicity in freshwater prawns.