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Development of novel polymorphic EST ‐ SSR markers in Californian abalone H aliotisrufescens and genetic analysis in wild and hatchery‐bred populations
Author(s) -
AguilarEspinoza Andrea,
ValderramaAravetalia,
Farlora Rodolfo,
Lafarga De la Cruz Fabiola,
GallardoEscárate Cristian
Publication year - 2014
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12141
Subject(s) - biology , microsatellite , inbreeding , fixation index , population , allele , locus (genetics) , genetics , haliotis discus , analysis of molecular variance , genetic variation , zoology , abalone , gene , fishery , demography , sociology
In this study, we describe the isolation and characterization of novel microsatellite markers for H aliotisrufescens derived from transcriptomepyrosequencing and heterologous loci obtained from expressed sequence tags previously reported for H . discus hannai . We were able to characterize 29 of 59 loci using 94 individuals from populations localized in M exico and C hile. For the Mexican population, the number of alleles per locus ranged from 2 to 11, while the observed and expected heterozygosities varied from 0 to 0.977 and 0.1 to 0.854 respectively. For the Chilean populations, the number of alleles per locus ranged from two to eight while the observed and expected heterozygosities varied from 0 to 0.8 and 0.1 to 0.847 respectively. The average inbreeding coefficient F is was 0.282 for the Mexican and 0.245 for the Chilean populations, while the polymorphic information content ranged from 0.590 to 0.927. Pairwise fixation index F st and analysis of molecular variance indicated a significantly high genetic difference between the two studied populations ( F st = 0.535). In addition, cross‐amplifications of 59 loci were also evaluated in H. fulgens , H. corrugate and H. discus hannai . These markers will constitute valuable tools for management strategies in aquaculture production and also for population genetic studies.