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The induction of Hsp70 synthesis by non‐lethal heat shock confers thermotolerance and resistance to lethal ammonia stress in the common carp, Cyprinus carpio (Linn)
Author(s) -
Sung Yeong Yik,
Liew Hon Jung,
Ambok Bolong Abol Munafi,
Abdul Wahid Mohd. Effendy,
MacRae Thomas H
Publication year - 2014
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12116
Subject(s) - cyprinus , biology , common carp , hsp70 , carp , heat shock protein , ammonia , lethal dose , gill , median lethal dose , heat stress , toxicology , biochemistry , zoology , toxicity , fishery , medicine , fish <actinopterygii> , gene
Exposure to heat‐shock protein (Hsp) stimulating factors induces Hsp accumulation and confers tolerance to lethal ammonia stress on the common carp Cyprinus carpio . This study investigated whether a non‐lethal heat shock bestowed similar protective effects against ammonia and induced thermotolerance, both thought to be rendered by increased amounts of Hsps. The 30‐min lethal temperature (30 min LHT ) and 1‐h lethal ammonia concentration (1 h LCT ) for this species occurred at 41°C and 14.2 mg/L NH 3 respectively. Heating juvenile carp (5 cm) from 28°C to 32, 34 and 38°C, with a subsequent 8‐h recovery period augmented tolerance to lethal heat and ammonia perturbation by two to threefold as compared with animals held at 28°C. Protection occurred in conjunction with Hsp70 accumulation in gills, substantiating the role of this Hsp in enhancing the stress tolerance of common carp.