Premium
Immunostimulatory effect of water soluble fraction of N yctanthes arbortristis leaves on the immune response in O reochromis mossambicus ( P eters)
Author(s) -
Devasree Laguduva Devajee,
Binuramesh Chandrasekaran,
Michael R. Dinakaran
Publication year - 2014
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12104
Subject(s) - aeromonas hydrophila , biology , lysozyme , oreochromis mossambicus , immunity , immune system , immunostimulant , tilapia , food science , immunology , fish <actinopterygii> , biochemistry , fishery
Use of immunostimulants as a prophylactic measure against diseases in fish is considered as an effective alternative to antibiotic use. Plant‐derived immunostimulants have recently received more attention, as most of them are cost‐effective and eco‐friendly. This study was carried out with the objective of evaluating the possible immunostimulatory activity of intraperitoneally injected water soluble fraction of N yctanthes arbortristis (an Indian medicinal plant) leaves on (i) specific immunity (antibody response), (ii) nonspecific immunity ( L ysozyme activity, ROS production) and (iii) functional immunity in terms of disease resistance against A eromonas hydrophila in O reochromis mossambicus . The results of the study showed that all the tested doses (i.e. 3.2, 16, 80 and 400 mg kg −1 ) of water soluble fraction of N yctanthes arbortristis leaves significantly enhanced both primary and secondary antibody responses to heat killed A. hydrophila . Significant enhancement in serum lysozyme activity by all the doses of water soluble fraction was observed on day 10. The lower doses of 3.2 and 16 mg kg −1 of water soluble fraction alone enhanced intracellular ROS production. Fish treated with both single and double dose of water soluble fraction showed maximum disease resistance. The highest dose (400 mg kg −1 ) of water soluble fraction exhibited highest relative per cent survival in both single dose and double dose groups.