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Stress Gene Expression in C rassostrea gigas (Thunberg, 1793) in response to experimental exposure to the toxic dinoflagellate P rorocentrum lima (Ehrenberg) Dodge, 1975
Author(s) -
RomeroGeraldo Reyna de Jesús,
HernándezSaavedra Norma Yolanda
Publication year - 2014
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12100
Subject(s) - dinoflagellate , biology , crassostrea , heat shock protein , hsp70 , algal bloom , gene expression , pacific oyster , heat shock , gene , superoxide dismutase , microbiology and biotechnology , oxidative stress , botany , biochemistry , ecology , phytoplankton , oyster , nutrient
Abstract Harmful algal blooms ( HAB s) are natural phenomena with different effects on the aquatic environment that affect both human economy and health. Several genomic studies have been done to characterize the effects of contaminants on C rassostrea gigas . However, oysters’ molecular response to HAB s exposure needs to be studied more. In this study, we challenged C . gigas with P rorocentrum lima, a diarrhoetic toxin producer, under controlled experimental conditions considering dinoflagellate density and exposure time (acute and sub‐chronic) as variables. The expression profile of six stress response genes was analysed by semiquantitative RT‐PCR : Glutamine synthetase ( GS ), Glutathione S‐transferase ( GST ), heat shock protein 70 ( HSP 70 ), and heat shock protein 90 ( HSP 90 ), CuZn superoxide dismutase ( SOD ) and melanogenic peroxidase ( POX ). The results revealed that these gene expressions depend on exposure time and cell concentration. In general, an increased expression was observed in all tested cell densities as immediate response to exposure (0–3 h); whereas lower expression levels than control were observed after 6 h. Treatment with 3,000 cells mL ‐1 promoted higher expression than control on most genes after 336 h of exposure. This is the first report providing information at molecular level on C . gigas response to dinoflagellate blooms.