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Effects of diets supplemented with either individual or combined probiotics, B acillus subtilis E20 and L actobacillus plantarum 7‐40, on the immune response and disease resistance of the mud crab, S cylla paramamosain ( E stampador)
Author(s) -
Yeh ShinnPing,
Chiu ChiuHsia,
Shiu YaLi,
Huang ZheLin,
Liu ChiuHung
Publication year - 2014
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12061
Subject(s) - lactobacillus plantarum , bacillus subtilis , biology , probiotic , immunity , immune system , microbiology and biotechnology , lactobacillus , food science , zoology , bacteria , immunology , fermentation , lactic acid , genetics
Two trials, including firstly, diets incorporating individual or combined species of probiotics, B acillus subtilis E20 and L actobacillus plantarum 7‐40, were fed to the mud crab, S cylla paramamosian, for 28 days to evaluate the growth, immunity and disease resistance, and secondly, the optimal level of B . subtilis E20 in the diet by tracking the growth, immunity and disease resistance after 28 days of feeding were analysed. No significant differences in growth, total haemocyte counts, respiratory bursts, superoxide dismutase, or glutathione peroxidase were found in the two trials. Crabs fed the B . subtilis E20‐containing diet (D20) had significantly increased phenoloxidase ( PO ) activity, phagocytic activity ( PA ) and disease resistance compared with crabs fed the control and the mixed probiotics diet ( MD ). The mortality of crabs fed the D20 was significantly lower than that of crabs fed the L . plantarum 7‐40‐containing diet. L . plantarum 7‐40 had a great inhibitory effect on the growth of B . subtilis E20, which may have led to the decreased probiotic effect of the MD . An analysis of the optimal level of B . subtilis E20 in the diet showed that crabs fed the B . subtilis E20‐containing diet at a level of 10 9–10 cfu kg −1 had better immune response of PO and PA , and disease resistance in the second trial.