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Rapid and accurate detection of Helicobacter pylori from biopsy specimens using loop‐mediated isothermal amplification
Author(s) -
Bakhtiari Somaye,
Hasanvand Banafsheh,
Pajavand Hamid,
Alvandi Amirhooshang,
Abiri Ramin
Publication year - 2019
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12953
Subject(s) - loop mediated isothermal amplification , gold standard (test) , helicobacter pylori , predictive value , polymerase chain reaction , positive predicative value , rapid urease test , biopsy , medicine , gastroenterology , biology , helicobacter pylori infection , dna , gene , genetics , biochemistry
Loop‐mediated isothermal amplification ( LAMP ) is a promising nucleic acid‐based assay for quick, accurate and cost‐effective diagnosis of many infectious agents. The purpose of this study was to assess the diagnostic value of LAMP for rapid and accurate detection of Helicobacter pylori in biopsy specimens. Patients suffering from one or several gastroduodenal disorders were enrolled in the study. Specificity, sensitivity, and the positive and negative predictive values of LAMP were compared with the gold standard result, which was the assembled result of culture, rapid urease test and polymerase chain reaction. Sensitivity, specificity, and the positive and negative predictive values of LAMP in comparison with the gold standard result were 100%, 30.76%, and 87.67% and 100% respectively [%95 CI ]. As the diagnostic value of LAMP is favourable, the method is an optimum technique for diagnosis the presence of H. pylori in different clinical and environmental samples.