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Porphyromonas gingivalis ‐induced inflammatory responses in THP 1 cells are altered by native and modified low‐density lipoproteins in a strain‐dependent manner
Author(s) -
Jayaprakash Kartheyaene,
Demirel Isak,
Khalaf Hazem,
Bengtsson Torbjörn
Publication year - 2018
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12860
Subject(s) - porphyromonas gingivalis , thp1 cell line , proteases , chemistry , microbiology and biotechnology , lipoprotein , inflammation , bacteroidaceae , strain (injury) , low density lipoprotein , foam cell , lipopolysaccharide , cell culture , biology , immunology , bacteria , biochemistry , cholesterol , enzyme , genetics , anatomy
Strong epidemiological evidence supports an association between cardiovascular and periodontal disease and furthermore, the periodontopathogen Porphyromonas gingivalis has been identified in blood and from atheromatous plaques. Blood exposed to P. gingivalis shows an increased protein modification of low‐density lipoprotein ( LDL ). In this study, we investigate the inflammatory responses of THP 1 cells incubated with P. gingivalis and the effects of native or modified LDL on these responses. Reactive oxygen species ( ROS ) and IL ‐1β were observed in THP 1 cells following infection with P. gingivalis ATCC 33277 and W50. Caspase 1 activity was quantified in THP 1 cells and correlated with IL ‐1β accumulation. Oxidized LDL (ox LDL ) induced IL ‐1β release and CD 36 expression on THP 1 cells. Modified LDL co‐stimulated with ATCC 33277 exhibited regulatory effects on caspase 1 activity, IL ‐1β release and CD 36 expression in THP 1 cells, whereas W50 induced more modest responses in THP 1 cells. In summary, we show that P. gingivalis is capable of inducing pro‐inflammatory responses in THP 1 cells, and native and modified LDL could alter these responses in a dose‐ and strain‐dependent manner. Strain‐dependent differences in THP 1 cell responses could be due to the effect of P. gingivalis proteases, presence or absence of capsule and proteolytic transformation of native and modified LDL .