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LAMP‐1 gene is overexpressed in high grade glioma
Author(s) -
Sarafian Victoria S.,
Koev Ilian,
Mehterov Nikolay,
Kazakova Maria,
Dangalov Krassimir
Publication year - 2018
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12856
Subject(s) - glioma , immunohistochemistry , cancer research , gene expression , autophagy , glycoprotein , gene , membrane glycoproteins , messenger rna , biology , tumor progression , microbiology and biotechnology , chemistry , apoptosis , immunology , biochemistry
High‐grade gliomas ( HGG ) are the most frequent brain tumors in adults. Glioblastoma multiforme ( GBM ) is their most aggressive form resistant to therapy. It was shown that inhibition of autophagy reduced GBM development and autophagy interfering agents are regarded as a new strategy to fight glioma cells. The lysosome‐associated membrane proteins ( LAMP s) display differential expression particularly in cancer. There are few data on their expression and especially on their molecular profile. The aim of the present study is to investigate the expression of LAMP ‐1 and LAMP ‐2 genes and proteins in HGG . Newly diagnosed patients with HGG and healthy controls were examined by immunohistochemistry and qPCR for both protein and mRNA levels of LAMP ‐1 and LAMP ‐2. The transcriptional activity of LAMP ‐1 in HGG was significantly higher compared to normal brain and to LAMP ‐2. The two glycoproteins were detected in the cytosol of tumor cells with varying intensity, LAMP ‐1 showing again enhanced expression. In conclusion, novel data on LAMP ‐1 overexpression in HGG are presented suggesting involvement of this gene and protein in cell adhesion and tumor progression. These findings might help the elucidation of the complex biological role of the multifunctional LAMP s proteins and to predict novel therapeutic targets in lysosomes.