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Challenges in the identification of methicillin‐resistant Staphylococcus argenteus by routine diagnostics
Author(s) -
Tunsjø Hege Smith,
Kalyanasundaram Sumana,
Charnock Colin,
Leegaard Truls Michael,
Moen Aina E. F.
Publication year - 2018
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12843
Subject(s) - staphylococcus aureus , identification (biology) , biology , microbiology and biotechnology , genome , staphylococcus , computational biology , whole genome sequencing , methicillin resistant staphylococcus aureus , staphylococcal infections , gene , genetics , bacteria , botany
Current clinical diagnostic procedures have shortcomings in the differentiation of Staphylococcus argenteus from Staphylococcus aureus . This article presents three cases of Staphylococcus argenteus obtained from clinical samples. The initial results from biochemical and molecular methods led to an incorrect identification of the isolates as methicillin‐resistant Staphylococcus aureus . Whole genome sequencing and real‐time PCR targeting the nonribosomal peptide synthetase gene led to their correct identification as methicillin‐resistant Staphylococcus argenteus . The study shows that real‐time PCR can be used to differentiate the two species in routine diagnostics. For purposes of identification based on whole genome sequencing, the Min ION portable sequencer is a simple and affordable approach which could be used by many laboratories.