Premium
Entrapment of LTB protein in alginate nanoparticles protects against Enterotoxigenic Escherichia coli
Author(s) -
Kordbacheh Emad,
Nazarian Shahram,
Hajizadeh Abbas,
Sadeghi Davood
Publication year - 2018
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12815
Subject(s) - enterotoxigenic escherichia coli , immunogenicity , adjuvant , chemistry , escherichia coli , microbiology and biotechnology , antigenicity , enterotoxin , antigen , toxin , chitosan , antibody , biochemistry , biology , gene , immunology
Vaccine delivery vehicles are just as important in vaccine efficiency. Through the progress in nanotechnology, various nanoparticles have been evaluated as carriers for these substances. Among them, alginate nanoparticles are a good choice because of their biodegradability, biocompatibility, ease of production, etc. In this study, feasibility of alginate nanoparticles ( NP s) such as recombinant LTB from Enterotoxigenic Escherichia coli ( ETEC ) carrier was investigated. To do this, the eltb gene was cloned and expressed in E. coli BL 21 ( DE 3) host cells, and a Ni‐ NTA column purified the protein. NP s were achieved through ion gelation method in the presence of LTB protein and CaCl 2 as the cross‐Linker and NP s were characterized physicochemically. Balb/C mice groups were immunized with LTB ‐entrapped NP s or LTB with adjuvant and immunogenicity was assessed by evaluating IgG titer. Finally, the neutralization of antibodies was evaluated by GM 1 binding and loop assays. LTB protein was expressed and efficiently entrapped into the alginate NP s. The size of NP s was less than 50 nm, and entrapment efficiency was 80%. Western blotting showed maintenance of the molecular weight and antigenicity of the released protein from NP s. Administration of LTB ‐entrapped NP s stimulated antibody responses in immunized mice. Immunization induced protection against LT toxin of ETEC in ileal loops and inhibits enterotoxin binding to GM 1‐gangliosides. Alginate NP s are also appropriate vehicle for antigen delivery purpose. Moreover because of their astonishing properties, they have the potential to serve as an adjuvant.