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Relationship between hepatic progenitor cells and stellate cells in chronic hepatitis C genotype 4
Author(s) -
Helal Thanaa El Sayed Ahmed,
Ehsan Nermine Ahmed,
Radwan Nehal Ahmed,
Abdelsameea Eman
Publication year - 2018
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12787
Subject(s) - hepatic stellate cell , progenitor cell , hepatic fibrosis , fibrosis , hepatitis c virus , hepatitis c , stem cell , pathology , immunology , biology , medicine , virus , microbiology and biotechnology
Hepatitis C virus ( HCV ) infection represents a major health problem in many areas of the world, especially Egypt. Hepatic progenitor cells ( HPC s) and hepatic stellate cells ( HSC s) have been implicated in fibrosis progression in chronic HCV . The aim of this study was to investigate the role of HPC s and HSC s in chronic HCV infection and the relationship between both cell types. This retrospective study was conducted on 100 chronic HCV patients. Immunohistochemistry was performed on liver tissue sections for cytokeratin 19 (progenitor cell markers), smooth muscle actin (stellate cell markers), matrix metalloproteinase‐9 ( MMP ‐9), and transforming growth factor beta ( TGF ‐ß). The necroinflammatory activity was significantly related to the number of isolated HPC s and TGF ‐ß expression (p = 0.003 and p = 0.001 respectively). Advanced stages of fibrosis showed significantly increase number of HPC s (p = 0.001), higher ratio of HSC s (p = 0.004), more expression of TGF ‐ß (p = 0.001) and MMP ‐9 (p = 0.001). There was a significant direct correlation between immunoexpression of HPC s and HSC s for isolated cells (r = 0.569, p = 0.001) and ductular reaction (r = 0.519, p = 0.001). Hepatic progenitor cells and stellate cells play a significant role in the development and progression of fibrosis in chronic HCV . More interestingly, the significant direct correlation between HPC s and HSC s suggests a synergistic interrelation.