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Association of Helicobacter pylori infection with Toll‐like receptor‐4 Thr399Ile polymorphism increased the risk of peptic ulcer development in North of Iran
Author(s) -
Tourani Mehdi,
Habibzadeh Maryam,
ShokriShirvani Javad,
Teymournejad Omid,
Mostafazadeh Amrollah,
Khafri Soraya,
Nouri Hamid Reza
Publication year - 2018
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12779
Subject(s) - helicobacter pylori , peptic ulcer , toll like receptor , immunology , medicine , gastroenterology , biology , immune system , innate immune system
Toll‐like receptor‐4 ( TLR 4) polymorphisms may influence host immune response against Helicobacter pylori ( H. pylori ). This study aimed to investigate whether TLR 4 polymorphisms are associated with H. pylori susceptibility and risk of peptic ulcer development or not. The TLR 4 + 3725 G/C polymorphism was studied using polymerase chain reaction with confronting two‐pair primers ( PCR – CTPP ). In addition, TLR 4 Asp299Gly and Thr399Ile polymorphisms were evaluated by PCR ‐restriction fragment length polymorphism ( RFLP ). There was no significant difference in TLR 4 + 3725 G/C and Asp299Gly genotype frequencies between non‐peptic ulcer ( NPUD ) and peptic ulcer ( PUD ) individuals in the context of peptic ulcer development and susceptibility to infection with H. pylori . Nevertheless, a significant association with increased risk for PUD development was observed for polymorphism TLR 4 Thr399Ile [odds ratio ( OR ) = 4.2; 95% confidence interval ( CI ) = 1.35–13.26; p = 0.01]. Correspondingly, TLR 4 Thr399Ile polymorphism was associated with H. pylori susceptibility ( OR = 0.27; 95% CI = 0.08–0.88; p = 0.04). In addition, TLR 4 Thr399Ile polymorphism increased 4.2‐fold, the risk of peptic ulcer development in individuals infected by H. pylori carrying CT + TT genotype. Our results showed that TLR 4 Thr399Ile polymorphism along with H. pylori infection may play critical roles in peptic ulcer development in North of Iran.