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Biofilm formation of beta‐hemolytic group G Streptococcus dysgalactiae subspecies equisimilis isolates and its association with emm polymorphism
Author(s) -
Ma JuiShan,
Chen SinYu,
Lo HsuehHsia
Publication year - 2017
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12746
Subject(s) - biofilm , microbiology and biotechnology , tryptic soy broth , virulence , subspecies , biology , streptococcus dysgalactiae , pathogen , bacteria , streptococcus , gene , genetics , streptococcus agalactiae , paleontology
Biofilm formation has been well known as a determinant of bacterial virulence. Group G Streptococcus dysgalactiae subspecies equisimilis ( SDSE ), a relevant pathogen with increasing medical importance, was evaluated for the biofilm‐forming potential. Microtiter plate assay was used to assess the most feasible medium for group G SDSE to form a biofilm. Among 246 SDSE isolates examined, 46.7%, 43.5%, 33.3%, and 26.4% of isolates showed moderate or strong biofilm‐forming abilities using tryptic soy broth ( TSB ), brain heart infusion broth ( BHI ), Todd‐Hewitt broth ( THB ), and C medium with 30 mM glucose ( CMG ), respectively. The addition of glucose significantly increased the biofilm‐forming ability of group G SDSE . FCT (fibronectin–collagen–T‐antigen) typing of SDSE was first undertaken and 11 FCT types were found. Positive associations of stG10.0 or negative associations of stG245.0 , stG840.0 , and stG6.1 with biofilm‐forming ability of SDSE were, respectively, found. This was the first investigation demonstrating biofilm‐forming potential in clinical group G SDSE isolates; also, some significant associations of biofilm‐forming ability with certain emm types were presented.