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Molecular characterization of nasal methicillin resistant Staphylococcus aureus isolates from workers of an automaker company in southeast Iran
Author(s) -
Sobhanipoor Mohammad Hossein,
Ahmadrajabi Roya,
Karmostaji Afsaneh,
Saffari Fereshteh
Publication year - 2017
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12732
Subject(s) - multilocus sequence typing , staphylococcus aureus , typing , microbiology and biotechnology , molecular epidemiology , methicillin resistant staphylococcus aureus , leukocidin , biology , mobile genetic elements , medicine , genotype , genetics , gene , bacteria , plasmid
Colonization of methicillin resistant Staphylococccus aureus ( MRSA ) can occur more commonly in healthy people who live in close together or are in close physical contact with each other. Having knowledge about the molecular characteristics of these strains provides considerable discernment into the epidemiology of this important microorganism. A total of 806 nasal swabs were collected from healthy workers of an automaker company in the southeast of Iran and were analyzed to detect MRSA isolates. Multilocus sequence typing ( MLST ), spa typing, and detection of staphylococcal cassette chromosome mec ( SCC mec ) were performed. The presence of genes encoding Panton‐Valentine Leukocidin ( PVL ) and Arginine Catabolic Mobile Element ( ACME ) were also investigated. Carriage rate of S. aureus was 20%. Among 10 identified MRSA , no acme was found while high prevalence of pvl (60%) was of great concern. Seven different spa types including five new ones were identified. The most frequent sequence type was the novel one; ST 3373 (n = 3), followed by each of ST 22, ST 88, ST 859 (n = 2) and ST 1955 (n = 1). MRSA isolates were clustered into two main clonal complexes; CC 22 (n = 6) and CC 88 (n = 4). Low genetic diversity with the dominance of CC 22, SCC mec IV was found. Distribution of previously found hospital‐associated MRSA was demonstrated among our isolates.