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Testing for high‐risk HPV in cervical and tonsillar paraffin‐embedded tissue using a cartridge‐based assay
Author(s) -
Virtanen Elina,
Laurila Pekka,
Hagström Jaana,
Nieminen Pekka,
Auvinen Eeva
Publication year - 2017
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12727
Subject(s) - concordance , medicine , colposcopy , cytology , histology , liquid based cytology , staining , cervical screening , pap test , pathology , cervical cancer screening , cervical cancer , cancer
This study evaluates the suitability of Xpert HPV (Cepheid, Sunnyvale, CA , USA ) test for cervical and tonsillar formalin‐fixed paraffin‐embedded ( FFPE ) tissue samples as compared to the tests currently used in diagnostics. Cervical biopsies and liquid cytology ( LC ) samples were collected from 48 women attending colposcopy. Biopsies were processed for histology and tested for hr HPV using Xpert HPV . LC samples were tested using Xpert and Hybrid Capture 2 ( HC 2; Qiagen, Hilden, Germany) tests. Also 29 archived tonsillar carcinoma samples were tested using Xpert, and the results were compared with histology and immunohistochemical p16 INK 4a (p16) staining. Among valid cervical LC samples 46.8% were hr HPV positive using Xpert test and 55.3% with HC 2. The sensitivity of Xpert was 84.6% as compared to HC 2, and overall test concordance was 91.5%. Test concordance between valid Xpert results from biopsies and LC samples was 84.6%. Among valid tonsillar samples 70.4% were hr HPV positive, and concordance of 96.3% was found between Xpert and p16 staining. To conclude, Xpert HPV test cartridge provides a convenient platform to test individual samples, including FFPE samples. Further studies are needed to establish whether test sensitivity is sufficient to reliably differentiate between hr HPV positive and hr HPV negative head and neck carcinomas.