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Prevalence and distribution of Chlamydia trachomatis genovars in Indian infertile patients: a pilot study
Author(s) -
Rawre Jyoti,
Dhawan Benu,
Malhotra Neena,
Sreenivas Vishnubhatla,
Broor Shobha,
Chaudhry Rama
Publication year - 2016
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12622
Subject(s) - chlamydia trachomatis , genotyping , infertility , biology , mycoplasma hominis , ureaplasma , restriction fragment length polymorphism , genotype , medicine , virology , mycoplasma , gynecology , microbiology and biotechnology , genetics , gene , pregnancy
To determine the prevalence and distribution of Chlamydia trachomatis genovars in patients with infertility by PCR ‐ RFLP and omp A gene sequencing. Prevalence of other etiological agents (viz., Ureaplasma spp. and Mycoplasma hominis ) were also assessed. Endocervical swabs were collected from 477 women and urine was collected from 151 men attending the Infertility Clinic. The samples were screened for C. trachomatis by cryptic plasmid, omp A gene and nested omp A gene PCR . Genotyping was performed by PCR ‐ RFLP and sequencing. Samples were screened for Ureaplasma spp. and M. hominis . The prevalence of C. trachomatis in infertile women and their male partners were 15.7% (75 of 477) and 10.0% (15 of 151) respectively. Secondary infertility was significantly associated with chlamydial infection. Genovar E was the most prevalent followed by genovar D and F. Twenty‐four C. trachomatis strains were selected for omp A gene sequencing. No mixed infection was picked. Variability in omp A sequences was seen in 50.0%. Both PCR ‐ RFLP and omp A gene sequencing showed concordant results. High prevalence of C. trachomatis in infertile couples warrants routine screening for C. trachomatis infection in all infertile couples. Genotyping of the omp A gene of C. trachomatis may be a valuable tool in understanding the natural history of C. trachomatis infection.