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The impact of langerin (CD207)+ dendritic cells and FOXP3+ Treg cells in the small bowel mucosa of children with celiac disease and atopic dermatitis in comparison to children with functional gastrointestinal disorders
Author(s) -
Vorobjova Tamara,
Ress Krista,
Luts Katrin,
Uibo Oivi,
Uibo Raivo
Publication year - 2016
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12552
Subject(s) - langerin , medicine , immunoglobulin e , immunology , immunohistochemistry , foxp3 , intestinal mucosa , atopic dermatitis , antibody , antigen , immune system , dendritic cell
In the present study we aimed to evaluate the impact of langerin ( CD 207)+ dendritic cells ( DC s) and FOXP 3+ Treg cells in the intestinal mucosa of children with celiac disease ( CD ) and atopic dermatitis ( AD ) in comparison to children with functional gastrointestinal disorders ( FGD ). Seventy‐five children (37 male, mean age 8.4 ± 4.8 years), who randomly underwent small bowel biopsy, were studied. The CD was diagnosed in 14 children, including five persons with concomitant AD (all positive for anti‐tissue transglutaminase IgA antibodies and with small bowel atrophy). Normal small bowel mucosa was found in eight patients with AD and in 53 patients with FGD . The sera of all patients were tested for total and specific IgE antibodies to food allergen panels. Staining for CD 11c+, langerin ( CD 207+) DC s, CD 4+, and FOXP 3+ Treg cells was performed on paraffin‐embedded sections of bioptates using immunohistochemistry. The density of CD 11c+ DC s, CD 4+, and FOXP 3+ Treg cells was higher in the CD patients compared to the AD and FGD patients (p = 0.02; p = 0.001). In AD , significantly higher density of CD 11c+ DC s was detected in patients positive for specific IgE to food allergen panels (p = 0.02). The FGD patients with elevated total IgE had increased density of langerin ( CD 207)+ DC s compared to the patients with normal total IgE levels (p = 0.01). The increased density of FOXP 3+ Treg cells, CD 4+, cells and CD 11c+ DC s was associated with CD but not with AD . The elevated level of total IgE or specific IgE to food allergens was associated with more pronounced expression of DC s, indicating a possible link between the presence of these cells in small bowel mucosa with elevated level of serum IgE.