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Influence of volatile organic compounds emitted by Pseudomonas and Serratia strains on Agrobacterium tumefaciens biofilms
Author(s) -
Plyuta Vladimir,
Lipasova Valentina,
Popova Alexandra,
Koksharova Olga,
Kuznetsov Alexander,
Szegedi Erno,
Chernin Leonid,
Khmel Inessa
Publication year - 2016
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12547
Subject(s) - agrobacterium tumefaciens , pseudomonas chlororaphis , microbiology and biotechnology , serratia , biofilm , pseudomonas fluorescens , biology , pseudomonas , bacteria , agrobacterium , virulence , transformation (genetics) , biochemistry , genetics , gene
The ability to form biofilms plays an important role in bacteria‐host interactions, including plant pathogenicity. In this work, we investigated the action of volatile organic compounds ( VOC s) produced by rhizospheric strains of Pseudomonas chlororaphis 449, Pseudomonas fluorescens B‐4117, Serratia plymuthica IC 1270 , as well as Serratia proteamaculans strain 94, isolated from spoiled meat, on biofilms formation by three strains of Agrobacterium tumefaciens which are causative agents of crown‐gall disease in a wide range of plants. In dual culture assays, the pool of volatiles emitted by the tested Pseudomonas and Serratia strains suppressed the formation of biofilms of A . tumefaciens strains grown on polycarbonate membrane filters and killed Agrobacterium cells in mature biofilms. The individual VOC s produced by the tested Pseudomonas strains, that is, ketones (2‐nonanone, 2‐heptanone, 2‐undecanone), and dimethyl disulfide ( DMDS ) produced by Serratia strains, were shown to kill A . tumefaciens cells in mature biofilms and suppress their formation. The data obtained in this study suggest an additional potential of some ketones and DMDS as protectors of plants against A. tumefaciens strains, whose virulence is associated with the formation of biofilms on the infected plants.