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Transurethral instillation with fusion protein MrpH.FimH induces protective innate immune responses against uropathogenic Escherichia coli and Proteus mirabilis
Author(s) -
Habibi Mehri,
Asadi Karam Mohammad Reza,
Bouzari Saeid
Publication year - 2016
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12523
Subject(s) - proteus mirabilis , microbiology and biotechnology , innate immune system , immunogenicity , escherichia coli , biology , fusion protein , adjuvant , immunity , immune system , immunology , recombinant dna , gene , biochemistry
Urinary tract infections ( UTI s) are among the most common infections in human. Innate immunity recognizes pathogen‐associated molecular patterns ( PAMP s) by Toll‐like receptors ( TLR s) to activate responses against pathogens. Recently, we demonstrated that MrpH.FimH fusion protein consisting of MrpH from Proteus mirabilis and FimH from Uropathogenic Escherichia coli ( UPEC ) results in the higher immunogenicity and protection, as compared with FimH and MrpH alone. In this study, we evaluated the innate immunity and adjuvant properties induced by fusion MrpH.FimH through in vitro and in vivo methods. FimH and MrpH.FimH were able to induce significantly higher IL ‐8 and IL ‐6 responses than untreated or MrpH alone in cell lines tested. The neutrophil count was significantly higher in the fusion group than other groups. After 6 h, IL ‐8 and IL ‐6 production reached a peak, with a significant decline at 24 h post‐instillation in both bladder and kidney tissues. Mice instilled with the fusion and challenged with UPEC or P. mirabilis showed a significant decrease in the number of bacteria in bladder and kidney compared to control mice. The results of these studies demonstrate that the use of recombinant fusion protein encoding TLR ‐4 ligand represents an effective vaccination strategy that does not require the use of a commercial adjuvant. Furthermore, MrpH.FimH was presented as a promising vaccine candidate against UTI s caused by UPEC and P. mirabilis .