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Studies on prevalence of biofilm associated genes and primary observation on sas X gene in clinical isolates of coagulase negative staphylococci (CoNS)
Author(s) -
Soumya Kandammuriyil Radhakrishnan,
Mathew Shiji,
Sugathan Sheela,
Mathew Jyothis,
Radhakrishnan Edayileveettil Krishnankutty
Publication year - 2016
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12510
Subject(s) - biofilm , staphylococcus epidermidis , microbiology and biotechnology , multiple drug resistance , staphylococcus haemolyticus , gene , biology , coagulase , doxycycline , antibiotics , antibiotic resistance , bacteria , staphylococcus , staphylococcus aureus , genetics
Coagulase negative staphylococci (Co NS ) are nosocomial pathogens that cause indwelling medical device associated infections due to its biofilm forming potential and multiple antibiotic resistance. The current study focused on species identification, antibiotic resistance profile and molecular basis of biofilm formation and attachment of Co NS isolated from clinical samples. Along with this, molecular screening for mec A and newly identified surface colonization protein encoded by sas X gene was also conducted. S. epidermidis (n = 19, 47%) was identified as the most prevalent Co NS species and very interestingly two biofilm forming, mec A positive S. epidermidis isolates were found to carry all the biofilm associated genes screened in this study, which indicates its potential to form the strong biofilm. Another novel observation of the study is the detection of sas X gene in one biofilm positive S. epidermidis isolate. The study also identified one doxycycline resistant mec A positive, multidrug resistant S. haemolyticus isolate. In conclusion, the study signifies the existence of multiple biofilm related genes, multidrug resistance and the presence of sas X gene among clinical isolates of Co NS .