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Dynamic and selective HERV RNA expression in neuroblastoma cells subjected to variation in oxygen tension and demethylation
Author(s) -
Hu Lijuan,
Uzhameckis Dmitrijs,
Hedborg Fredrik,
Blomberg Jonas
Publication year - 2016
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12494
Subject(s) - neuroblastoma , cell culture , demethylation , housekeeping gene , microbiology and biotechnology , biology , oxygen tension , rna , gene expression , transcription (linguistics) , gene , chemistry , biochemistry , oxygen , dna methylation , genetics , organic chemistry , linguistics , philosophy
We studied HERV expression in cell lines after hypoxia, mitogenic stimulation, and demethylation, to better understand if hypoxia may play a role in ERV activation also within the nervous system, as represented by neuroblastoma cell lines. The level of RNA of four human ERV groups ( HERV s) ( HERVE , I/T, H, and W), and three housekeeping genes, of different cell lines including A549, COS ‐1, Namalwa, RD ‐L and Vero‐E6, as well as human neuroblastoma cel l lines SH ‐ SY 5Y, SK ‐N‐ DZ , and SK ‐N‐ AS were studied using reverse transcription and real‐time quantitative PCR ( QPCR ). During the course of recovery from hypoxia a pronounced and selective activation of RNA expression of HERVW ‐like sequences, but not of HERVE , I/T, H, and three housekeeping genes, was found in the neuroblastoma cell lines, most pronounced in SK ‐N‐ DZ . In the SK ‐N‐ DZ cell line, we also tested the expression of HERV s after chemical treatments. HERVW ‐like sequences were selectively upregulated by 5‐azacytidine, a demethylating agent. Some HERVW loci seem especially responsive to hypoxia and demethylation. HERV expression in neuroblastoma cells is selectively and profoundly influenced by some physiological and chemical stimuli.