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Analytical specificity and sensitivity of the novel dual‐target GeneProof  Neisseria gonorrhoeae PCR kit for detection of N. gonorrhoeae
Author(s) -
Golparian Daniel,
Hellmark Bengt,
Unemo Magnus
Publication year - 2015
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12440
Subject(s) - neisseria gonorrhoeae , nucleic acid amplification tests , microbiology and biotechnology , neisseria , neisseriaceae , biology , 16s ribosomal rna , virology , gene , bacteria , chlamydia trachomatis , genetics , antibiotics
Detection of Neisseria gonorrhoeae relies increasingly on nucleic acid amplification tests ( NAAT s). The specificity of many gonococcal NAAT s has been suboptimal and supplementary testing remains recommended in Europe and several additional countries. The novel dual‐target GeneProof  Neisseria gonorrhoeae PCR kit, targeting porA pseudogene and 16S rRNA gene, showed a high specificity and sensitivity when isolates of non‐gonococcal Neisseria and related species (n = 144), and gonococci (n = 104) were tested. However, rare gonococcal porA mutants were only detected in the 16S rRNA gene target and two non‐gonococcal isolates showed a low‐level cross‐reactivity in the 16S rRNA gene target. The detection limit for both targets was 1.5 copies per reaction.

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