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Clonal dissemination of multilocus sequence type ST 15 KPC ‐2‐producing K lebsiella pneumoniae in B ulgaria
Author(s) -
Markovska Rumyana,
Stoeva Temenuga,
Schneider Ines,
Boyanova Lyudmila,
Popova Valentina,
Dacheva Daniela,
Kaneva Radka,
Bauernfeind Adolf,
Mitev Vanyo,
Mitov Ivan
Publication year - 2015
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12433
Subject(s) - multilocus sequence typing , klebsiella pneumoniae , microbiology and biotechnology , biology , rapd , plasmid , clone (java method) , colistin , replicon , tigecycline , genotype , genetics , escherichia coli , antibiotics , genetic diversity , gene , population , medicine , environmental health
A total of 36 consecutive clinical and two fecal‐screening carbapenem‐resistant Klebsiella pneumoniae isolates from two Bulgarian university hospitals (Varna and Pleven) were investigated. Susceptibility testing, conjugation experiments, and plasmid replicon typing were carried out. Beta‐lactamases were characterized by isoelectric focusing, PCR , and sequencing. Clonal relatedness was investigated by RAPD and multilocus sequence typing ( MLST ). Most of the isolates demonstrated multidrug resistance profile. Amikacin and tigecycline retained good activity with susceptibility rates of 95 and 87%, respectively. The resistance rate to colistin was 63%. Six RAPD ‐ and MLST ‐types were identified: the dominating MLST ‐type was ST 15 (27 isolates), followed by ST 76 (six isolates), and ST 1350 (two isolates). ST 101, ST 258, and ST 151 were detected once. All except one of the K. pneumoniae produced KPC ‐2, mostly in combination with CTX ‐M‐15, while for one isolate ( ST 101) the enzymes OXA ‐48 and CTX ‐M‐14 were found. All KPC ‐2‐producing transconjugants revealed the presence of Inc FII plasmid. The OXA ‐48‐ and CTX ‐M‐14‐producing isolate showed the presence of L/M replicon type. The dissemination of KPC ‐2‐producing K.pneumoniae in Bulgaria is mainly due to the sustained spread of successful ST 15 clone and to a lesser extent of ST 76 clone. This is the first report of OXA ‐48 producing ST 101 K. pneumoniae in Bulgaria.