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Elevated levels of peripheral blood CD 14 bright CD 16 + and CD 14 dim CD 16 + monocytes may contribute to the development of retinopathy in patients with juvenile onset type 1 diabetes
Author(s) -
RybaStanisławowska Monika,
Myśliwska Jolanta,
Juhas Ulana,
Myśliwiec Małgorzata
Publication year - 2015
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12419
Subject(s) - cd16 , cd14 , monocyte , flow cytometry , diabetes mellitus , immunology , type 1 diabetes , context (archaeology) , medicine , endocrinology , biology , antigen , cd8 , cd3 , paleontology
The study aimed to analyze the CD 14 bright CD 16 + and CD 14 dim CD 16 + monocyte subsets in juvenile‐onset complication‐free diabetes mellitus type 1 in the context of their association with microvascular complications. 61 children with type 1 diabetes and 30 healthy individuals were enrolled in a study. CD 14 bright CD 16 + and CD 14 dim CD 16 + monocytes were quantified in peripheral blood by means of flow cytometry. At the time of sampling blood glucose concentration was taken along with biochemical measurement of renal function, CRP and glycosylated hemoglobin. The Spearman's correlations were used to compare the relationship between CD 16 + monocyte subsets and the clinical parameters that can predict the development of microangiopathies. The flow cytometric analysis of monocyte subsets in peripheral blood of analyzed subjects revealed that the numbers of CD 14 bright CD 16 + and CD 14 dim CD 16 + monocytes were significantly higher in patients with type 1 diabetes than in the healthy individuals. As to the relationship between CD 16 + monocyte subsets and the clinical parameters that can predict development of microangiopathies, it was shown that both CD 16 + subsets were associated with increased risk of retinopathy development, defined as retinopathy development value. Elevated levels of intermediate CD 14 bright CD 16 + and non‐classical CD 14 dim CD 16 + monocytes predict development of diabetic retinopathy in patients with type 1 diabetes.