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Detection of Chlamydia trachomatis , Ureaplasma urealyticum and Mycoplasma hominis in infertile Bulgarian men with multiplex real‐time polymerase chain reaction
Author(s) -
OuzounovaRaykova Vessela,
Rangelov Simeon,
Ouzounova Iordanka,
Mitov Ivan
Publication year - 2015
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12391
Subject(s) - mycoplasma hominis , ureaplasma urealyticum , chlamydia trachomatis , polymerase chain reaction , mycoplasma , infertility , medicine , ureaplasma , chlamydia , biology , microbiology and biotechnology , gynecology , immunology , pregnancy , gene , genetics , biochemistry
The effect of Chlamydia trachomatis , Ureaplasma urealyticum and Mycoplasma hominis over the sperm quality is still unclear. The aim of this study was to determine their prevalence in infertile Bulgarian men. A total of 281 men were examined by applying mRT ‐ PCR . The registered prevalence was as follows: C. trachomatis – 13.9%, U. urealyticum – 19.2%, M. hominis – 9.9%. Co‐infection was established in eight swabs. This first in Bulgaria to study for detection of chlamydia and mycoplasmas in infertile men by mRT‐PCR demonstrates higher prevalence of the tested microorganisms in the infertile group toward the control one.