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Comparison of four molecular methods to type Salmonella Enteritidis strains
Author(s) -
Campioni Fábio,
PitondoSilva André,
Bergamini Alzira M.M.,
Falcão Juliana P.
Publication year - 2015
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12367
Subject(s) - multiple loci vntr analysis , pulsed field gel electrophoresis , multilocus sequence typing , subtyping , salmonella enteritidis , biology , typing , variable number tandem repeat , microbiology and biotechnology , molecular epidemiology , salmonella enterica , intergenic region , genetics , salmonella , genotype , gene , bacteria , genome , computer science , programming language
This study compared the pulsed‐field gel electrophoresis ( PFGE ), enterobacterial repetitive intergenic consensus‐ PCR ( ERIC ‐ PCR ), multilocus variable‐number of tanden‐repeat analysis ( MLVA ), and multilocus sequence typing ( MLST ) methods for typing 188 Salmonella Enteritidis strains from different sources isolated over a 24‐year period in Brazil. PFGE and ERIC ‐ PCR were more efficient than MLVA for subtyping the strains. However, MLVA provided additional epidemiological information for those strains. In addition, MLST showed the Brazilian strains as belonging to the main clonal complex of S . Enteritidis, CC 11, and provided the first report of two new ST s in the S. enterica database but could not properly subtype the strains. Our results showed that the use of PFGE or ERIC ‐ PCR together with MLVA is suitable to efficiently subtype S . Enteritidis strains and provide important epidemiological information.