Premium
Dysregulation of circulating CD 4+ CXCR 5+ T cells in type 2 diabetes mellitus
Author(s) -
Wang Qijin,
Zhai Xiao,
Chen Xiao,
Lu Jiancan,
Zhang Yaping,
Huang Qin
Publication year - 2015
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12330
Subject(s) - medicine , diabetes mellitus , abdominal obesity , endocrinology , cxcr5 , obesity , body mass index , type 2 diabetes mellitus , flow cytometry , type 2 diabetes , immunology , inflammation , metabolic syndrome , chemokine , chemokine receptor
Type 2 diabetes mellitus (T2 DM ) is characterized by a chronic low‐grade inflammatory state. Follicular helper T cells (Tfh) play critical roles in inducing B‐cell activation and producing various cytokines, whereas circulating CD 4+ CXCR 5+ T cells ( CT fh) may act as a counterpart to measure Tfh cell disorders. In this study, we investigated whether Tfh could be involved in the development of T2 DM by assessing CT fh in peripheral blood. CT fh and it subtypes were determined by measuring CD 3, CD 4, CXCR 5, CXCR 3, and CCR 6 in 68 T2 DM patients and 60 healthy controls using flow cytometry. Results showed that proportion of CT fh in the peripheral CD 4+ T cells was significantly increased in T2 DM patients (8.5 ± 0.5%) than in controls (4.5 ± 0.3%) (p < 0.001). Further study revealed that the balance of CT fh subtypes was greatly dysregulated, in which percentage of Th17 subtype was significantly increased in patients. Investigating the correlation between CT fh and risk factors of T2 DM demonstrated that proportion of CT fh were significantly elevated in patients with body mass index ( BMI ) over 24.0 (p = 0.005). Interestingly, patients with abdominal obesity had further increase in CT fh than those without abdominal obesity. This study suggests the involvement of CT fh in T2 DM , especially in T2 DM ‐related obesity.