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Cyclin D1, p16 INK 4A and p27 Kip1 in pancreatic adenocarcinoma: assessing prognostic implications through quantitative image analysis
Author(s) -
Georgiadou Despoina,
Sergentanis Theodoros N.,
Sakellariou Stratigoula,
Filippakis George M.,
Zagouri Flora,
Vlachodimitropoulos Dimitris,
Psaltopoulou Theodora,
Lazaris Andreas C.,
Patsouris Efstratios,
Zografos George C.
Publication year - 2014
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12289
Subject(s) - quantitative analysis (chemistry) , physics , adenocarcinoma , cyclin d1 , nuclear physics , chemistry , medicine , cell cycle , cancer
The prognostic significance of cyclin D1, p16 INK 4A and p27 Kip1 expression has been documented in several human malignancies; however, their prognostic potential in pancreatic adenocarcinoma is still unclear. This study aimed to assess the correlation of the aforementioned molecules with clinicopathological parameters and prognosis. Sixty patients with pancreatic ductal adenocarcinoma underwent surgical resection at a single institution; immunohistochemical staining of the studied markers was quantified by Ιmage analysis system. Cyclin D1 overexpression was positively associated with grade, neural infiltration and vascular invasion, whereas p27 positively correlated with age. Higher cyclin D1 expression indicated poorer survival (adjusted HR = 9.75, 95% CI : 1.48–64.31, p = 0.018, increment: one unit in H‐score), whereas a marginal trend toward an association between p16 positivity and improved survival was observed (adjusted HR = 0.58, 95% CI : 0.32–1.05, p = 0.072 regarding positive vs negative cases). No significant association with overall survival was noted regarding p27. In conclusion, cyclin D1 overexpression and possibly p16 loss of expression in pancreatic adenocarcinoma seem to be adverse prognostic factors, whereas p27 expression did not seem to possess such prognostic properties. Further validation of the present findings in studies encompassing larger samples seems to be needed.