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Rab27A regulates exosome secretion from lung adenocarcinoma cells A549: involvement of EPI 64
Author(s) -
Li Wenhai,
Hu Yunsheng,
Jiang Tao,
Han Yong,
Han Guoliang,
Chen Jiakuan,
Li Xiaofei
Publication year - 2014
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12261
Subject(s) - exosome , microvesicles , secretion , a549 cell , exocytosis , microbiology and biotechnology , biology , extracellular , extracellular vesicle , chemistry , cell , biochemistry , microrna , gene
Exosomes are small membrane vesicles secreted into the extracellular compartment by exocytosis. The unique composition of exosomes can be transported to other cells which allow cells to exert biological functions at distant sites. However, in lung cancer, the regulation of exosome secretion was poorly understood. In this study, we employed human lung adenocarcinoma A549 cells to determine the exosome secretion and involved regulation mechanism. We found that Rab27A was expressed in A549 cells and the reduction of Rab27A by Rab27A‐specific sh RNA could significantly decrease the secretion of exosome by A549 cells. EPI 64, a candidate GAP that is specific for Rab27, was also detected in A549 cells. By pull‐down assay, we found that EPI 64 participated in the exosome secretion of A549 cells by acting as a specific GAP for Rab27A, not Rab27B. Overexpression of EPI 64 enhanced exosome secretion. Taken together, in A549 cells, EPI 64 could regulate the exosome secretion by functioning as a GAP specific for Rab27A.