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Genotypic characteristics of multidrug‐resistant E scherichia coli isolates associated with urinary tract infections
Author(s) -
Cao Xiaoli,
Zhang Zhifeng,
Shen Han,
Ning Mingzhe,
Chen Junhao,
Wei Hongxia,
Zhang Kui
Publication year - 2014
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12260
Subject(s) - integron , microbiology and biotechnology , pulsed field gel electrophoresis , biology , genotype , multiple drug resistance , escherichia coli , multilocus sequence typing , typing , clone (java method) , ceftazidime , antibiotic resistance , drug resistance , pseudomonas aeruginosa , virology , antibiotics , bacteria , genetics , gene
E scherichia coli is an important pathogen involved in community‐acquired urinary tract infections ( CA ‐ UTI s). In this study, we analyzed the prevalence of frequently occurring genes and the distribution of integrons in 51 multidrug‐resistant ( MDR ) E . coli isolates associated with CA ‐ UTI s. The clonality of these strains was investigated by phylogrouping, multi‐locus sequence typing, and pulsed‐field gel electrophoresis ( PFGE ). All these strains were found to produce two or more resistance determinants, ceftazidime‐hydrolyzing CTX ‐M‐type extended‐spectrum β‐lactamases ( ESBL s) and plasmid‐mediated quinolone resistance determinants were the most prevalent (92.2% and 51.0%, respectively). A sulfhydryl variable‐61‐producing E . coli strain was identified for the first time in C hina. The prevalence of class 1 integrons was 54.9%, class 2 integrons were detected in three isolates but no isolate contained a class 3 integron. Phylogenetic group D was the dominant, observed in 70.6% of the isolates. PFGE analysis revealed a high level of diversity. Twenty‐four distinctive sequence types ( ST s) including four major ST s ( ST 648, ST 224, ST 38, and ST 405) were identified. To our knowledge, this is the first report on the characterization of MDR E . coli isolates associated with CA ‐ UTI s in C hina; our results suggest that an MDR D‐ ST 648 clone producing CTX ‐M‐ ESBL s has emerged as a major clone in the community setting.